Background Knowledge
Important information about biology and biotechnology to know.
To understand this course, you should be familiar with:
    1.
    What a microarray is and what it does
    2.
    What is gene expression?
Essentially microarrays are cool devices that let us measure how much each gene is "expressed" in a given sample, such as a patient's cells.
For example, let's say there's a gene called ABCD. Hypothetically, ABCD is "expressed" as a protein that leads to cancer somehow. We'd expect ABCD to be more "expressed" in people with cancer.
We can use a microarray to confirm our hypothesis by measuring how much ABCD expression there is in cancer patients vs. non-cancer people.
What exactly is gene expression? Gene expression is how much a gene is expressed. Genes are expressed when they are transcribed to RNA and then made into protein. Microarrays can measure the amounts of RNA for each gene and from that information, you can infer how much the gene is expressed.
Gene Expression flowchart. Credit: https://www.azolifesciences.com/article/A-Guide-to-Understanding-Gene-Expression.aspx
Don't understand this explanation? Check out the free recommended resources below.
Please review the following resources if you are unfamiliar with the central dogma and biotechnology.
What is the 'Central Dogma'?
yourgenome
4.1: Central Dogma of Molecular Biology
Biology LibreTexts
Introduction to DNA Microarrays
Bitesize Bio
DNA Microarray
Last modified 5mo ago
Export as PDF
Copy link